SUMMER SCHOOL 2: “FISHing Bacteria”

!Important!

Great news! A new date has been set for DelNAM's SS2. This event will take place on the 4th-6th of May 2021. Look forward to seeing you there.

The event may undergo some changes due to limitations imposed by the COVID-19 pandemic!

About

The 2nd DelNAM Summer School will take place on from the 4th to the 6th of May 2021 and is organised by the Faculty of Engineering of the University of Porto.

This summer school will include theoretical sessions and hands-on practical sessions on the FISH technique and its applications. The practical sessions will include the design of oligonucleotides to target bacteria, standard FISH on planktonic and mixed biofilms, as well as variants including the application of liposomes, microfluidics and spectral image analysis.

Registration is free of charge, since it is financed by the European Comission's H2020 programme through the DelNAM project. There is a limit of registrations for the practical sessions; registrations will be accepted by order of submission.

 

Organizing  Committee:

Nuno F. Azevedo, Rita S. Santos, Laura Cerqueira, Andreia Azevedo, Nuno Guimarães, Célia Rodrigues, Carina Almeida

Staff:

Mariana Gomes, Beatriz Magalhães, Luís Moreira, Sara Pereira, Jontana Alkja

Programme (tentative)

Greenwich Mean Time (GMT)

DAY 1: Theoretical Sessions

8h30 – 9h

Registration

9h - 9h15

Welcome Session

9h15 – 9h45

Chemically-modified nucleic acid mimics (NAMS) for improved RNA targeting

Jesper Wengel (University of Southern Denmark, Denmark)

9h45 – 10h15

Application of nucleic acid mimics to target microorganisms

Nuno F. Azevedo (Faculty of Engineering of the University of Porto, Portugal)

10h15 – 10h45

Coffee-break

10h45 – 11h15

µFISH

Govind Kaigala (IBM Research - Zurich, Switzerland)

11h15 – 11h45

CLASI-FISH to study microbial community structure across multiple spatial scales

Alex Valm (State University of New York at Albany, USA)

11h45 – 12h15

smFISH

Zach Hensel (ITQB, Portugal)

12h15 – 13h30

Lunch

13h30 – 14h

Aptamers to target microorganisms

Jeffrey J. DeStefano (University of Maryland, USA)

14h – 14h30

Nanoscopy tools to image and dynamically interact with molecular cell processes

Paolo Bianchini (Istituto Italiano di Tecnologia, Italy)

14h30 – 15h

Mobile Microscopy

Hatice Ceylan Koydemir (UCLA, USA)

15h – 15h30

Coffee-break

15h30 – 16h45

Oral Communications from selected abstracts

Rapid fluorescence based assays for in situ enumeration of microorganisms - Linda Mezule (Riga Technical University, Latvia)

Disinfection of non-cultivable microorganisms in water supply systems - Viktorija Denisova (Riga Technical University, Latvia)

PNA-FISH approach in Bacterial Vaginosis: from diagnosis to unveiling etiology - Joana Castro (University of Minho, Portugal)

Characterization of bacterial vaginosis-associated multi-species communities by multiplex PNA FISH - Aliona Rosca (University of Minho, Portugal)

Localization of plant pathogenic viruses and bacteria, and endosymbiotic bacteria in plants and insect vectors using FISH methods - Murad Ghanim (Volcani Center, Israel)

16h45 – 17h15

Introduction to the Practical Sessions

18h30 – 23h

Social Activity (if possible, given the current pandemic situation)

DAY 2: Theoretical and Practical Sessions

9h – 9h30

Taking FISH from lab to market

Laura Cerqueira (BIOMODE, Portugal)

9h30 - 10h

Drug Development – From bench to bedside

Ricardo Cunha (Blueclinical, Portugal)

10h – 10h30

Coffee-break

10h30 – 13h

Design of oligonucleotides to target bacteria

Nuno Guimarães (Faculty of Engineering of the University of Porto, Portugal)

13h – 14h

Lunch

14h – 17h

Hands-on session

- overview of the practical work

- prepare and treat samples

14h30 – 15h

DelNAM GA Meeting (for consortium members only)

15h – 16h

DelNAM Advisory Board Meeting (for consortium members only)

DAY 3: Practical Sessions

9h – 13h

Hands-on session

- Standard FISH

- FISH on mixed biofilms

- FISH using liposomes

13h – 14h

Lunch

14h – 17h

Hands-on session

- Results analysis: microscopy visualization, spectral image analysis and microfluidics

17h – 17h30

Evaluation of the summer school

Venue

Venue: Faculty of Engineering of the University of Porto. Department of Chemical Engineering, Room E007.

Address: s/n, R. Dr. Roberto Frias, 4200-465 Porto.

Travel and Accommodation

How to get to Porto, Portugal

 

By Plane

The nearest airport is the Francisco Sá Carneiro Airport in Porto, about 17Km away from the city centre (RECOMMENDED).

Alternatively, you can fly to the Lisbon Portela Airport in Lisbon, about 300Km away from Porto, and then take another flight to the Francisco Sá Carneiro Airport or a train to São Bento station.

By Metro or Bus

From the Francisco Sá Carneiro Airport to Porto’s City Centre

The easiest way is to take the metro at the airport (E line – lilac) and get off at Trindade. Here, you should take another train (D line – yellow) and get off at Aliados. The entire travel will take around 35 minutes. You can buy metro tickets at the airport.

Alternatively, you can take the bus 601 and get off at Cordoaria, though it takes longer (50min). You can check bus timetables here and buy the tickets inside the bus.

How to get to the Venue

 

By Metro or Bus

The easiest way is to take the metro at the Aliados or São Bento (D line – yellow) and get off at Pólo Universitário or IPO. You can buy metro tickets on the station.

Where to Stay

 

Hotel Aliados ***

Situated in Porto's city centre.
ADDRESS: R. Elísio de Melo 27, 4000-196 Porto
CONTACT: +351 22 200 4853
WEBSITE

Hotel ibis Porto São João **

Situated close to the venue.
ADDRESS: Rua Dr Placido Costa, 4200-450, Porto, Portugal
CONTACT: +351 22 551 3100
WEBSITE

Eurostars Oporto ****

Situated close to the venue.
ADDRESS: Rua do Mestre Guilherme Camarinha, nº212, 4200-537 Porto, Portugal
CONTACT: +351 22 507 2090
WEBSITE

Registration

Don't forget, participation is free of charge! The Summer School is financed by the European Comission's H2020 programme through the DelNAM project.
You can choose whether you want to attend only the theoretical sessions (4th and 5th of May) or both the theoretical and practical sessions (4th-6th of May). There is a limit of registrations for the practical sessions, registrations will be accepted by order of submission.
Please also select whether you wish to take part in the social event on the 4th of May (taking place depending on the evolution of the pandemic situation).


Registrations are closed.

Abstract Submission

To present your work at the Workshop you must be registered in the event.
Abstracts must be written in English. Please refer to the Abstract Rules and the Abstract Template files for Submission Rules. The deadline for abstract submissions is the 15th of February 2020.


Submissions are closed.

Contact Information

Rita S. Santos (Project Manager)

Faculty of Engineering of the University of Porto

PHONE: +351 969 757 873
E-MAIL: ritasantos@fe.up.pt

Nuno F. Azevedo (Project Leader)

Faculty of Engineering of the University of Porto

PHONE: +351 969 754 859
E-MAIL: nazevedo@fe.up.pt