The 2nd DelNAM Summer School will take place on from the 4th to the 6th of May 2021 and is organised by the Faculty of Engineering of the University of Porto.

This summer school will include theoretical sessions and hands-on practical sessions on the FISH technique and its applications. The practical sessions will include the design of oligonucleotides to target bacteria, standard FISH on planktonic and mixed biofilms, as well as variants including the application of liposomes, microfluidics and spectral image analysis.

The theoretical sessions will take place fully ONLINE on the first day of the event (May 4th), while the practical sessions will happen IN PERSON at FEUP on the following days (May 5th and 6th).

Registration is free of charge, since it is financed by the European Comission's H2020 programme through the DelNAM project. There is a limit of registrations for the practical sessions; registrations will be accepted by order of submission.

 

Organizing  Committee:

Nuno F. Azevedo, Rita S. Santos, Laura Cerqueira, Andreia Azevedo, Nuno Guimarães, Célia Rodrigues, Carina Almeida

Staff:

Mariana Gomes, Beatriz Magalhães, Luís Moreira, Sara Pereira, Jontana Alkja

DAY 1: Theoretical Sessions (Online)

9h - 9h15

Welcome Session

9h15 – 9h45

Chemically modified nucleic acid mimics (NAMS) for improved RNA targeting

Jesper Wengel (University of Southern Denmark, Denmark)

9h45 – 10h15

Nucleic acid mimic applications in fluorescence in situ hybridization

Nuno F. Azevedo (Faculty of Engineering of the University of Porto, Portugal)

10h15 – 10h45

Break

10h45 – 11h15

Micro fluorescence in situ hybridization (μFISH) using a microfluidic scanning probe

Govind Kaigala (IBM Research - Zurich, Switzerland)

11h15 – 11h45

Single-molecule mRNA FISH in bacteria

Zach Hensel (ITQB, Portugal)

11h45 – 12h30

Nanoscopy tools to image and dynamically interact with molecular cell processes

Paolo Bianchini (Istituto Italiano di Tecnologia, Italy)

12h30 – 13h30

Lunch Break

13h30 – 14h45

Oral Communications from selected abstracts

Rapid fluorescence-based assays for in situ enumeration of microorganisms - Linda Mezule (Riga Technical University, Latvia)

Disinfection of non-cultivable microorganisms in water supply systems - Viktorija Denisova (Riga Technical University, Latvia)

PNA-FISH approach in Bacterial Vaginosis: from diagnosis to unveiling etiology - Joana Castro (University of Minho, Portugal)

Characterization of bacterial vaginosis-associated multi-species communities by multiplex PNA FISH - Aliona Rosca (University of Minho, Portugal)

Localization of plant pathogenic viruses and bacteria, and endosymbiotic bacteria in plants and insect vectors using FISH methods - Murad Ghanim (Volcani Center, Israel)

14h45 – 15h15

Break

15h15 – 15h45

Aptamers to target microorganisms

Jeffrey J. DeStefano (University of Maryland, USA)

15h45 – 16h15

Practical and Theoretical Aspects of CLASI-FISH imaging applied to microbial communities

Alex Valm (State University of New York at Albany, USA)

16h15 – 16h45

Mobile Microscopy

Hatice Ceylan Koydemir (UCLA, USA)

16h45 – 17h

Break

17h – 17h30

Taking FISH from lab to market

Laura Cerqueira (LEPABE, formerly BIOMODE, Portugal)

17h30 - 18h

Drug Development – From bench to bedside

Ricardo Cunha (Blueclinical, Portugal)

DAY 2: Practical Sessions

9h – 9h45

Introduction to the Practical Sessions

9h45 – 11h30

Design of oligonucleotides to target bacteria

Nuno Guimarães (Faculty of Engineering of the University of Porto, Portugal)

11h30 – 12h

Break

12h – 13h30

Hands-on session

- sample preparation

13h30 – 14h30

Lunch Break

14h30 – 17h

Hands-on session

- sample preparation

- liposome preparation

14h30 – 15h

DelNAM GA Meeting (for consortium members only)

15h – 16h

DelNAM Advisory Board Meeting (for consortium members only)

DAY 3: Practical Sessions

How to get to Porto, Portugal

 

By Plane

The nearest airport is the Francisco Sá Carneiro Airport in Porto, about 17Km away from the city centre (RECOMMENDED).

Alternatively, you can fly to the Lisbon Portela Airport in Lisbon, about 300Km away from Porto, and then take another flight to the Francisco Sá Carneiro Airport or a train to São Bento station.

The easiest way is to take the metro at the airport (E line – lilac) and get off at Trindade. Here, you should take another train (D line – yellow) and get off at Aliados. The entire travel will take around 35 minutes. You can buy metro tickets at the airport.

Alternatively, you can take the bus 601 and get off at Cordoaria, though it takes longer (50min). You can check bus timetables here and buy the tickets inside the bus.

How to get to the Venue

 

The easiest way is to take the metro at the Aliados or São Bento (D line – yellow) and get off at Pólo Universitário or IPO. You can buy metro tickets on the station.

Registrations are closed.